α 53bp1 (Cell Signaling Technology Inc)
Cell Signaling Technology Inc is a verified supplier 
Cell Signaling Technology Inc manufactures this product 
96
Structured Review
Cell Signaling Technology Inc
α 53bp1
α 53bp1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 680 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+53bp1/53BP1+Antibody/pm42894850-227-31-33
Average 96 stars, based on 680 article reviews
α 53bp1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 680 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+53bp1/53BP1+Antibody/pm42894850-227-31-33
Average 96 stars, based on 680 article reviews
α 53bp1 - by Bioz Stars,
2026-10
96/100 stars
Images
Related Articles
Control:Article Title: Nuclear DAB2IP regulates DNA replication initiation through activating PLK1-mediated HBO1 phosphorylation Article Snippet: .. The following primary antibodies were used in the present study: anti-DAB2IP, anti-MCM2, anti-MCM2-pSer40/41, anti-cyclin A (Bethyl Laboratories, Montgomery, TX, USA); anti-HBO1, anti-PARP, anti-cleaved caspase 3, anti-histone H3, anti-H3K14Ac, anti-p-SP motif, anti-p-TP motif, Immunohistochemistry:Article Title: STN1 facilitates metastasis by promoting transcription of EMT-activator ZEB1 in pancreatic cancer Article Snippet: .. Following commercial antibodies were used in this study. clone number, catalogue number and dilutions were shown in turn: anti-STN1 (ployclonal, Ab89250, 1:500, Abcam), anti-GAPDH (OTI2D9, TA-08, 1:5000, ZSGB-BIO), anti-HA (HA-7, H3663, 1:2000, Sigma-Aldrich), anti-FLAG (M2, F3165, 1:2000, Sigma-Aldrich), anti-E-cadherin (24E10, 3157T, 1:1000, Cell Sighnaling Technology), anti-Vimentin (V9, sc-6260, 1:1000, Santa Cruz), anti-E-cadherin(OTI1B8, ZM-0092, for IHC, ZSGB-BIO ), anti-Vimentin (EP21, ZA-0511, for IHC, ZSGB-BIO), anti-ZEB1 (D80D3, 3396T, 1:1000, Cell Signaling Technology, for WB), Western Blot:Article Title: STN1 facilitates metastasis by promoting transcription of EMT-activator ZEB1 in pancreatic cancer Article Snippet: .. Following commercial antibodies were used in this study. clone number, catalogue number and dilutions were shown in turn: anti-STN1 (ployclonal, Ab89250, 1:500, Abcam), anti-GAPDH (OTI2D9, TA-08, 1:5000, ZSGB-BIO), anti-HA (HA-7, H3663, 1:2000, Sigma-Aldrich), anti-FLAG (M2, F3165, 1:2000, Sigma-Aldrich), anti-E-cadherin (24E10, 3157T, 1:1000, Cell Sighnaling Technology), anti-Vimentin (V9, sc-6260, 1:1000, Santa Cruz), anti-E-cadherin(OTI1B8, ZM-0092, for IHC, ZSGB-BIO ), anti-Vimentin (EP21, ZA-0511, for IHC, ZSGB-BIO), anti-ZEB1 (D80D3, 3396T, 1:1000, Cell Signaling Technology, for WB), other:Article Title: The EHMT2-MBLAC2 axis suppresses ribosomal DNA transcription in response to nucleolar DNA damage Article Snippet: HeLa, U2OS, GES1 and HEK293T cells were obtained from ATCC and SW480 cells were obtained from Servicebio. Article Title: ZBP1 antagonizes MRE11-mediated DNA end resection and confers synthetic lethality to PARP inhibition in ovarian cancer. Article Snippet: ZBP1, a classic pattern recognition receptor (PRR), has been implicated in regulating programmed cell death and the innate immune response.. However, the role of ZBP1 in the nucleus remains largely undefined.. Here, we found that nuclear ZBP1 localizes to the site of DNA double-stranded breaks (DSBs) following DNA damage and impairs homologous recombination (HR) repair through its interaction with MRE11. Incubation:Article Title: TopBP1 biomolecular condensates as a new therapeutic target in advanced-stage colorectal cancer Article Snippet: Revelation was carried out with ECL Clarity (Bio-Rad, #170-5061) or ECL select (Bio-Rad, #1705062) according to the manufacturer’s instructions. .. Cells were grown on coverslips and after incubation with the corresponding drugs (see above) for 2 hr, the soluble fraction of cells was removed during the pre-extraction step in Cytoskeleton buffer ‘CSK’ (PBS containing 0.2% Triton X-100) at 4°C for 60 s. Cells were then fixed with 4% PFA/PBS at RT for 20 min. Nonspecific epitopes were saturated with a blocking solution (5% BSA/PBS) at RT for 30 min. For immunostaining, anti-TopBP1 (Santa Cruz Biotechnology, #sc-271043, diluted at 1/100), Article Title: Defects in the DNA Damage Response of Patient-derived Endometriosis Stromal Cells Article Snippet: Cells were washed with 1× PBS and were permeabilized/blocked for 1 hour with 1× PBS containing 10% donkey serum (Equitech-Bio Inc., USA, SD30-0500), 1% BSA (Fisher Bioreagents, USA, BP9703-100), and 0.1% triton (Millipore Sigma, USA, X-100). .. Cells were washed with 1× PBS and incubated in primary antibodies [anti-vimentin (Biotechne, USA, MAB2105; 1:500), anti-γH2AX S139 (Cell Signaling, USA, 2577S; 1:500), anti-pATM S1981 (GeneTex, USA, GTX132146; 1:500), Blocking Assay:Article Title: TopBP1 biomolecular condensates as a new therapeutic target in advanced-stage colorectal cancer Article Snippet: Revelation was carried out with ECL Clarity (Bio-Rad, #170-5061) or ECL select (Bio-Rad, #1705062) according to the manufacturer’s instructions. .. Cells were grown on coverslips and after incubation with the corresponding drugs (see above) for 2 hr, the soluble fraction of cells was removed during the pre-extraction step in Cytoskeleton buffer ‘CSK’ (PBS containing 0.2% Triton X-100) at 4°C for 60 s. Cells were then fixed with 4% PFA/PBS at RT for 20 min. Nonspecific epitopes were saturated with a blocking solution (5% BSA/PBS) at RT for 30 min. For immunostaining, anti-TopBP1 (Santa Cruz Biotechnology, #sc-271043, diluted at 1/100), Immunostaining:Article Title: TopBP1 biomolecular condensates as a new therapeutic target in advanced-stage colorectal cancer Article Snippet: Revelation was carried out with ECL Clarity (Bio-Rad, #170-5061) or ECL select (Bio-Rad, #1705062) according to the manufacturer’s instructions. .. Cells were grown on coverslips and after incubation with the corresponding drugs (see above) for 2 hr, the soluble fraction of cells was removed during the pre-extraction step in Cytoskeleton buffer ‘CSK’ (PBS containing 0.2% Triton X-100) at 4°C for 60 s. Cells were then fixed with 4% PFA/PBS at RT for 20 min. Nonspecific epitopes were saturated with a blocking solution (5% BSA/PBS) at RT for 30 min. For immunostaining, anti-TopBP1 (Santa Cruz Biotechnology, #sc-271043, diluted at 1/100), Cell-Signaling:Article Title: TopBP1 biomolecular condensates as a new therapeutic target in advanced-stage colorectal cancer Article Snippet: Revelation was carried out with ECL Clarity (Bio-Rad, #170-5061) or ECL select (Bio-Rad, #1705062) according to the manufacturer’s instructions. .. Cells were grown on coverslips and after incubation with the corresponding drugs (see above) for 2 hr, the soluble fraction of cells was removed during the pre-extraction step in Cytoskeleton buffer ‘CSK’ (PBS containing 0.2% Triton X-100) at 4°C for 60 s. Cells were then fixed with 4% PFA/PBS at RT for 20 min. Nonspecific epitopes were saturated with a blocking solution (5% BSA/PBS) at RT for 30 min. For immunostaining, anti-TopBP1 (Santa Cruz Biotechnology, #sc-271043, diluted at 1/100), |